Analysis of Superoxide Dismutase (OsSOD) Gene Expression using qRT- PCR, its Morphophysiological Characters and Path Analysis in Rice Variety IR64 Under Aluminum Stress
Miftahul Huda Fendiyanto, Rizky Dwi Satrio, Mentari Pratami, Isna Arofatun Nikmah, Nastiti Intan Permata Sari, I Dewa Ketut Kerta Widana, Didi Darmadi · International Journal of Agriculture and Biology · 2021
Abstract
Rice treated with aluminum (Al) can produce active oxygen species (AOS). The existence of AOS can cause the rice plants to become morpho-physiologically damaged and eventually cause a decrease in productivity. The AOS reductive compounds are physiologically responded by plants using antioxidant compounds, one of which is the superoxide dismutase enzyme (SOD). Therefore, this research aimed to analyze FeSOD, MnSOD and CuZnSOD gene expressions in IR64 rice variety as information for selection and genetic improvement of rice to overcome national food security. We tested the root architecture, analyzed SOD gene expression and performed path analysis tests. The results showed that rice treated with aluminum (Al) showed dramatically increased CuZnSOD and FeSOD gene expression, while MnSOD gene expression was relatively the same under Al stress or normal conditions. The level of expression of the two genes positively correlated with physiological characteristics such as chlorophyll and root length. Thus, these two genes can be used as markers in studying Al tolerance in Indica cv. rice. IR64.